Table 1.
The Apoptotic Potential of Curcumin (Cur), Caffeine (Caff), and Thymoquinone (TQ).
| (A) HCT116a | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Control | 8.4 ± 1.9 | ||||||||
| Dox | 18.8 ± 5.2 | ||||||||
| Cur | Caff | TQ | |||||||
| 5 µM | 15 µM | 30 µM | 5 mM | 10 mM | 15 mM | 25 µM | 50 µM | 75 µM | |
| Proliferating | 7.6 ± 1.3 | 9.6 ± 1.7 | 97.1 ± 1.3 | 9.6 ± 2.9 | 15.7 ± 4.3 | 16.2 ± 4.8 | 10.8 ± 1.8 | 12.8 ± 1.0 | 21.7 ± 2.8 |
| Dox-treated | 25.4 ± 0.7* | 69.5 ± 9.7* | 97.9 ± 0.8 | 19.9 ± 1.2 | 36.5 ± 7.4ɸ | 48.0 ± 6.1* | 20.3 ± 2.5 | 21.9 ± 0.7 | 31.6 ± 2.7ɸ |
| (B) MCF7b | |||||||||
| Control | 8.4 ± 2.1 | ||||||||
| Dox | 27.9 ± 7.5 | ||||||||
| Cur | Caff | TQ | |||||||
| 5 µM | 15 µM | 30 µM | 5 mM | 10 mM | 15 mM | 25 µM | 50 µM | 75 µM | |
| Proliferating | 9.4 ± 2.3 | 11.0 ± 1.8 | 93.9 ± 1.5 | 10.8 ± 5.4 | 10.9 ± 4.5 | 11.9 ± 3.1 | 7.7 ± 1.9 | 14.6 ± 2.2 | 37.4 ± 0.3 |
| Dox-treated | 35.1 ± 2.9+ | 40.1 ± 2.6+ | 98.3 ± 1.7 | 45.9 ± 6.1* | 45.5 ± 1.4* | 49.0 ± 2.4* | 31.2 ± 4.9* | 84.0 ± 0.3* | 85.6 ± 5.1* |
Data are expressed as mean (%) of annexin-V ± SD in HCT116 cells. Two-way analysis of variance (ANOVA) was used followed by the Bonferroni posttest, *P < .001, ɸP < .01. The P values represent the comparison between Dox-treated with a natural compound (Cur, Caff, or TQ) and proliferating cells treated with the same compounds without Dox. The mean value of control proliferative and control Dox refers to the mean of their values as shown in Figures 4 to 6.
Data are expressed as mean (%) of annexin-V ± SD in MCF7 cells. Two-way ANOVA was used followed by the Bonferroni posttest, *P < .001, +P < .05. The P value values represent the comparison between Dox-treated with a natural compound (Cur, Caff, or TQ) and proliferating cells treated with the same compounds without Dox. The mean value of control proliferative and control Dox refers to the mean of their values as shown in Figures 4 to 6.