Table 1. Biophysical properties of Kv2.1 variants singly or co-expressed with WT.
Singly expressed variants with at least 10pA/pF are included. Values that differ from the respective WT condition are shown in red (Dunnett’s T3 multiple comparisons), and n = the number of cells recorded.
| Configurations or variants | Voltage-dependence of activation | Voltage-dependence of inactivation | ||
|---|---|---|---|---|
| V½ | k | V½ | k | |
| WT | −4.6 ± 1.0 | 13.0 ± 0.4 | −35.5 ± 2.6 | 7.5 ± 0.6 |
| S202F |
24.3 ± 3.8 p<0.001 (n=14) |
16.4 ± 1.7 p>0.189 (n=14) |
2.7 ± 1.2 p<0.001 (n=30) |
8.8 ± 0.4 p<0.001 (n=30) |
| R312H |
28.1 ± 1.5 p<0.001 (n=21) |
18.2 ± 1.0 p<0.001 (n=21) |
−0.1 ± 2.3 p<0.001 (n=18) |
8.5 ± 0.7 p<0.001 (n=18) |
| R325Q | −5.0 ± 2.5 p>0.884 (n=18) |
14.8 ± 1.2 p>0.804 (n=18) |
−39.6 ± 1.8 p> 0.704 (n=17) |
7.1 ± 0.4 p>0.083 (n=17) |
| R325W |
−23.4 ± 3.9 p<0.001 (n=22) |
17.7 ± 1.2 p>0.033 (n=22) |
−58.0 ± 1.4 p<0.001 (n=25) |
5.7 ± 0.2 p<0.001 (n=25) |
| S457R | −13.3 ± 1.4 p>0.050 (n=25) |
12.0 ± 0.7 p>0.952 (n=25) |
−44.2 ± 1.7 p>0.543 (n=38) |
6.5 ± 0.2 p>0.418 (n=38) |
| Y533X |
13.0 ± 3.6 p<0.001 (n=17) |
18.3 ± 1.4 p<0.001 (n=17) |
−45.7 ± 2.6 p>0.313 (n=26) |
6.8 ± 0.6 p>0.200 (n=26) |
| WT+WT | −7.3 ± 0.9 | 12.9 ± 0.9 | −39.6 ± 0.6 | 6.3 ± 0.1 |
| WT+S202F | 1.3 ± 3.1 p>0.184 (n=25) |
22.2 ± 0.8 p<0.001 (n=25) |
−29.6 ± 1.7 p<0.001 (n=54) |
10.4 ± 0.4 p<0.001 (n=54) |
| WT+T210K | −10.8 ± 1.3 p>0.395 (n=44) |
13.1 ± 0.5 p>0.999 (n=44) |
−46.2 ± 1.3 p<0.001 (n=34) |
6.3 ± 0.2 p>0.999 (n=34) |
| WT+T210M | −6.9 ± 1.7 p>0.999 (n=29) |
12.9 ± 0.9 p>0.999 (n=29) |
−43.3 ± 2.4 p>0.917 (n=25) |
6.5 ± 0.3 p>0.996 (n=25) |
| WT+Y274fsX | −7.1 ± 4.0 p>0.999 (n=18) |
14.5 ± 1.1 p>0.949 (n=18) |
−40.2 ±1.6 p>0.999 (n=65) |
5.7 ± 0.2 p>0.380 (n=65) |
| WT+R306C | −15.9 ± 3.7 p>0.435 (n=11) |
15.3 ± 2.2 p>0.989 (n=11) |
−44.9 ± 3.2 p>0.847 (n=22) |
9.0 ± 0.6 p<0.003 (n=22) |
| WT+R312C | −9.1 ± 1.3 p>0.989 (n=35) |
13.4 ± 0.5 p>0.999 (n=35) |
−42.0 ± 1.3 p>0.850 (n=27) |
6.0 ± 0.1 p>0.946 (n=27) |
| WT+R312H | 0.1 ± 2.7 p>0.198 (n=31) |
14.3 ± 0.7 p>0.776 (n=31) |
−38.7 ± 1.5 p>0.999 (n=78) |
7.7 ± 0.4 p>0.010 (n=78) |
| WT+R325Q | −10.7 ± 1.4 p>0.527 (n=43) |
12.8 ± 0.6 p>0.999 (n=43) |
−43.4 ± 1.7 p>0.531 (n=32) |
5.9 ± 0.2 p>0.957 (n=32) |
| WT+R325W |
−14.3 ± 1.7 p<0.011 (n=39) |
16.1 ± 1.0 p>0.077 (n=39) |
−51.9 ± 1.5 p<0.001 (n=35) |
5.9 ± 0.2 p>0.957 (n=35) |
| WT+E330D | −12.4 ± 2.3 p>0.529 (n=18) |
15.3 ± 1.4 p>0.825 (n=18) |
−40.4 ± 2.3 p>0.999 (n=52) |
7.4 ± 1.0 p>0.987 (n=52) |
| WT+W370R | −3.5 ± 3.3 p>0.986 (n=12) |
16.4 ± 1.8 p>0.663 (n=12) |
−38.9 ± 2.9 p>0.999 (n=26) |
6.9 ± 0.4 p>0.813 (n=26) |
| WT+V378A | −7.5 ± 1.4 p>0.999 (n=29) |
13.3 ± 0.5 p>0.999 (n=29) |
−41.4 ± 1.2 p>0.976 (n=32) |
5.6 ± 0.2 p>0.159 (n=32) |
| WT+V378L | −5.8 ± 4.8 p>0.999 (n=20) |
16.0 ± 1.2 p>0.298 (n=20) |
−46.4 ± 1.9 p>0.022 (n=61) |
7.5 ± 0.3 p<0.003 (n=61) |
| WT+G381R | −9.4 ± 2.7 p>0.999 (n=27) |
14.2 ± 1.0 p>0.916 (n=27) |
−41.2 ±1.4 p>0.995 (n=62) |
6.3 ± 0.5 p>0.999 (n=62) |
| WT+P385T | −6.7 ± 4.1 p>0.999 (n=11) |
14.9 ± 1.2 p>0.850 (n=11) |
−40.6 ± 2.1 p>0.999 (n=43) |
6.9 ± 0.5 p>0.958 (n=43) |
| WT+K391N | −9.4 ± 1.2 p>0.947 (n=44) |
12.6 ± 0.4 p>0.999 (n=44) |
−41.4 ± 1.2 p>0.977 (n=39) |
5.9 ± 0.1 p>0.492 (n=39) |
| WT+F416L | −3.1 ± 3.7 p>0.988 (n=16) |
15.6 ± 1.1 p>0.388 (n=16) |
−39.5 ± 1.6 p>0.999 (n=48) |
7.3 ± 0.4 p>0.175 (n=48) |
| WT+S457R |
−18.9 ± 3.0 p<0.019 (n=22) |
14.1 ± 1.0 p>0.989 (n=22) |
−42.0 ± 1.6 p>0.960 (n=42) |
5.9 ± 0.2 p>0.959 (n=42) |
| WT+Y533X | −11.6 ± 1.7 p>0.384 (n=42) |
14.8 ± 0.8 p>0.468 (n=42) |
−43.0 ± 1.4 p>0.432 (n=65) |
6.0 ± 0.2 p>0.998 (n=65) |