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. 2020 Feb 14;38:e2018351. doi: 10.1590/1984-0462/2020/38/2018351

Table 1. PCR product size in base pairs for exons 7, 11, and 12 of the phenylalanine hydroxylase gene, mutation, endonuclease used, and fragments generated in base pairs.

Exon PCR product Mutations Endonuclease Fragment(s) after digestion - wild Fragment(s) (bp) after digestion - mutated
7 263 R261Q a HinfI 116/147 263
R252W a Aval 86/177 263
R261X b DdelI 263 115/148
11 301 V388M a BsaAI 114/187 301
IVS10nt-11G>A b DdelI 301 78/223
12 238 R408W b Styl 238 97/141

adestroys restriction site; bcreates restriction site.