Figure 3.

Complement hemolytic activity in serum of multiple injured pigs via the alternative pathway (AH-50). Pigs received multiple injury, followed by femoral nailing of the fracture (poly nail, circle) or conventional reaming of the fracture (poly conv, rhombus). Control animals underwent sham-procedure (sham, square). AH-50 was measured at 4 h (A,B) and 6 h (C,D) after trauma. For AH-50 lysis curves, a serial dilution of the serum from 4 h after trauma (A) and 6 h after (C) was performed (x-axis, dilution in 1/x) and the respective OD values of the serum were determined at 415 nm (y-axis, OD). Dotted line represents OD value of 50% lysis of sensitized sheep erythrocytes. AH-50 values (1/x) of 4 h after trauma (B) and 6 h after trauma (D). Results are presented as mean ± SEM. n = 5. *p ≤ 0.05.