Skip to main content
. 2020 Jan 31;11:64. doi: 10.3389/fimmu.2020.00064

Figure 5.

Figure 5

Effects of C3a and C5a on human cardiomyocytes (CMs) in vitro. Cellular staining of the C5a receptor 1 (C5aR1) in human CMs in presence of PBS (control) or in presence of C5a (A). Human CMs were treated for 3 h with either PBS or with 10 ng/ml C5a and C5aR1 was stained (green). Cell nuclei were stained with Hoechst (blue) and cytoskeleton was stained with rhodamine-labeled phalloidin (red). Caspase-3/7 activity (counts/s) in human CMs in presence of C3a (light gray) and C5a (dark gray) (B). Frequency of calcium signals (s) of human CMs in presence of C3a and C5a (C). Mean decay time (s) of calcium peaks of human CMs in presence of C5a (D). Basal respiration oxygen consumption rate (OCR in pmol/m in /Norm. unit) of human CMs in presence of C3a and C5a (E). Spare respiratory capacity oxygen consumption rate (OCR in pmol/m in /Norm. unit) of human CMs in presence of C3a and C5a (F). Results are presented as mean ± SEM. n = 6. *p ≤ 0.05.