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. 2020 Mar;26(3):361–371. doi: 10.1261/rna.070318.119

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© 2020 Brumwell et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 5. — Endogenous RPS24 short and long transcript variants, and exogenous protein isoforms associate with polysomes. (A) Using transcript variant-specific RPS24 primers, short and long variants were amplified via RT-PCR from polysome fractions with either cycloheximide, or EDTA as a control to dissociate polysomes. (B) Polysomes were isolated from two cell lines each stably expressing one of the FLAG-tagged RPS24 protein isoforms (eS24) using cycloheximide and EDTA. Western blots were performed using anti-FLAG, or β-Actin as a control protein not associated with polysomes. Experiments performed in U87MG glioblastoma in normoxia (21% O₂). See Supplemental Table S1 for primer sequences.