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High-temperature IVT does not affect antisense dsRNA by-product formation. (A) Native gel electrophoresis analysis of IVT reactions on 512B DNA template using wild-type T7 (37°C) with/without RNase III treatment. (B) dsRNA immunoblot with J2 antibody on IVT reactions (crude and purified) with 512B template. (C) Native gel electrophoresis analyses and dsRNA immunoblot analysis of 512B IVT reactions conducted with TsT7-1 at 37°C versus 50°C.
