Table 1.
Some properties of active and dormant Mtb cells.
Characteristic | Active cells | Dormant cells 4.5 months (D1) |
Dormant cells 13 months (D2) |
---|---|---|---|
CFU, cells/ml | (5 ± 2) × 107 | (1 ± 0.5) × 104 | 0 |
MPN, cells/ml | 1.2 × 108-mean 3.7 × 107-low* 4.2 × 108-high* |
9.3 × 107-mean 1.8 × 107-low* 4.2 × 108-high* |
1.5 × 106-mean 4.5 × 105-low* 4.2 × 106-high* |
H3- Uracil inc. rate, CPM/mg wet cell weight | 3,623 ± 52 | 0 | 0 |
cAMP, pmol/mg wet cell weight | 124 ± 4 | 2 ± 1 | 0 |
14C-asparigine inc. rate CPM/mg wet cell weight | 340 ± 20 | 0 | 0 |
DCPIP reduction, nmol DCPIP min−1 mg−1 wet cell weight | 0.11 ± 0.03 | 0.016 ± 0.003 | 0 |
Protein amounts in cytosol, mg/g wet cell weight | 3.6 ± 0.2 | 2.13 ± 0.2** | 2.55 ± 0.2** |
Protein amounts in membrane fraction, μg/g wet cell weight | 76.2 ± 5 | 39 ± 5** | 20.7 ± 5** |
95 percent confidence intervals.
Dormant cells were 10 times washed before protein determination.