Table 2.
Proteins found in 10 most abundant spots in D2 proteome profile.
| Fraction | Product | Gene | Place, active | Place, D2 |
|---|---|---|---|---|
| Membrane | Probable iron-regulated elongation factor TU Tuf (EF-TU) | Rv0685 | 2 | 1 |
| Cytosol | Probable 5-methyltetrahydropteroyltriglutamate–homocysteine methyltransferase MetE (methionine synthase, vitamin-B12 independent isozyme) | Rv1133c | 85 | 1 |
| Cytosol | Transketolase Tkt (TK) | Rv1449c | N/D | 1 |
| Cytosol | Malate synthase G GlcB | Rv1837c | 134 | 1 |
| Cytosol | Catalase-peroxidase-peroxynitritase T KatG | Rv1908c | 85 | 1 |
| Cytosol | Probable chaperone protein DnaK (heat shock protein 70) (heat shock 70 kDa protein) (HSP70) | Rv0350 | 5 | 2 |
| Cytosol | 60 kDa chaperonin 2 GroEL2 (protein CPN60-2) (GroEL protein 2) (65 kDa antigen) (heat shock protein 65) (cell wall protein A) (antigen A) | Rv0440 | 3 | 2 |
| Membrane | Conserved 35 kDa alanine rich protein | Rv2744c | 9 | 2 |
| Cytosol | Maltokinase Mak | Rv0127 | N/D | 3 |
| Membrane | Isoniazid inductible gene protein IniB | Rv0341 | N/D | 3 |
| Membrane | Conserved protein | Rv1232c | 41 | 3 |
| Cytosol | Glutamine synthetase GlnA1 (glutamine synthase) (GS-I) | Rv2220 | 122 | 3 |
| Cytosol | Probable adenosylhomocysteinase SahH (S-adenosyl-L-homocysteine hydrolase) (adohcyase) | Rv3248c | N/D | 3 |
| Cytosol | 10 kDa chaperonin GroES (protein CPN10) (protein GroES) (BCG-a heat shock protein) (10 kDa antigen) | Rv3418c | N/D | 4 |
| Membrane | Probable bifunctional protein acetyl-/propionyl-coenzyme A carboxylase (alpha chain) AccA3: biotin carboxylase + biotin carboxyl carrier protein (BCCP) | Rv3285 | 83 | 5 |
| Membrane | 60 kDa chaperonin 1 GroEL1 (protein CPN60-1) (GroEL protein 1) | Rv3417c | 48 | 6 |
| Cytosol | Probable iron-regulated phosphoenolpyruvate carboxykinase [GTP] PckA (phosphoenolpyruvate carboxylase) (PEPCK)(pep carboxykinase) | Rv0211 | 130 | 7 |
| Membrane | Probable short-chain type oxidoreductase | Rv0484c | N/D | 7 |
| Membrane | Possible ketoacyl reductase | Rv1544 | N/D | 7 |
| Membrane | DNA-binding protein HU homolog HupB (histone-like protein) (HLP) (21-kDa laminin-2-binding protein) | Rv2986c | N/D | 7 |
| Cytosol | Probable acetohydroxyacid synthase IlvX (acetolactate synthase) | Rv3509c | 83 | 8 |
| Cytosol | Probable NAD(P) transhydrogenase (subunit alpha) PntAa [first part; catalytic part] (pyridine nucleotide transhydrogenase subunit alpha) (nicotinamide nucleotide transhydrogenase subunit alpha) | Rv0155 | 94 | 9 |
| Membrane | Probable succinate dehydrogenase [iron-sulfur subunit] (succinic dehydrogenase) | Rv0247c | 12 | 9 |
| Cytosol | Probable succinyl-CoA synthetase (beta chain) SucC (SCS-beta) | Rv0951 | 119 | 9 |
| Cytosol | Probable phosphoglycerate kinase Pgk | Rv1437 | 94 | 9 |
| Membrane | Probable catechol-O-methyltransferase | Rv1703c | N/D | 9 |
| Membrane | Conserved protein | Rv3205c | N/D | 9 |
| Membrane | Probable O-antigen/lipopolysaccharide transport ATP-binding protein ABC transporter RfbE | Rv3781 | N/D | 9 |
| Membrane | Probable short-chain type dehydrogenase/reductase | Rv0148 | N/D | 10 |
| Membrane | Periplasmic superoxide dismutase [Cu-Zn] SodC | Rv0432 | 5 | 10 |
| Cytosol | Probable citrate synthase I GltA2 | Rv0896 | N/D | 10 |
In the columns marked as “place,” proteins were arranged according to their spot density. See also M&M. Proteins with increased abundance in D2 cells vs. active cells (place for active cells proteome minus place for D2 > 10) are highlighted including proteins which were virtually absent in the active cells proteome (marked as “ND”).