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. 2020 Feb 7;11:138. doi: 10.3389/fimmu.2020.00138

Figure 5.

Figure 5

rFVIIIFc inhibits anti-Ig F(ab)2 induced calcium flux in B cells. (A) Calcium flux assay stimulation conditions and their hypothesized outcomes. (B) Representative graph of calcium flux assay results. (C) Peak calcium flux and (D) area under the curve for B cells stimulated with anti-Ig (10 μg/ml), anti-Ig F(ab)2 (10 μg/ml), anti-Ig F(ab)2 + FVIII (10 + 11.4 μg/ml) and anti-Ig F(ab)2 + rFVIIIFc (10 + 14.7 μg/ml). n = 3/condition. Errors bars represent SD. *p < 0.05, ***p < 0.001. Inline graphic, anti-Ig; Inline graphic, anti-Ig F(ab)2; Inline graphic, anti-Ig F(ab)2 + FVIII; Inline graphic, anti-Ig F(ab)2 + rFVIIIFc.