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. 2020 Jan 24;10:3123. doi: 10.3389/fimmu.2019.03123

Figure 2.

Figure 2

NK cells isolated from PBMCs by NK cell enrichment or CD3/CD19 depletion show similar transduction efficiencies when transduced with VSV-G pseudotyped lentiviral CD19-CAR particles. (A) Vectofusin-1 based transduction of differently isolated NK cells was performed with VSV-G pseudotyped lentiviral CD19-CAR particles at shown MOIs. NK cells from four different donors were used (n = 4). (B) Mean fluorescence intensities (MFI) of CD19-CAR in transduced cells. Data show average MFIs of CD19-CAR+ cells transduced with depicted MOIs as shown in (A). (C) CD19-CAR expression of CD16+ and CD16− NK cell subpopulations. CD19-CAR expression of CD16+ and CD16− NK cell subpopulations of transduced cells depicted in (A) are shown (n = 4). Shown are mean values + SD. Statistical analysis was performed using two-tailed student's paired t-test. No significant differences were observed between analyzed groups transduced with the same MOI as p-values were >0.05.