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. 2020 Jan 24;10:3123. doi: 10.3389/fimmu.2019.03123

Figure 7.

Figure 7

Cytokine secretion and cytotoxic capacity of CD19-CAR-NK cells generated by RD114-TR pseudotyped lentiviral CD19-CAR particles. (A) NT-NK cells and lentiviral/RD114-TR CD19-CAR-NK cells were cocultured with Sup-B15 cells at an E:T ratio of 1:1 for 4 h. As controls, NK cells and Sup-B15 cells were cultured alone. Levels of GM-CSF, TNF-α, MIP-1α, and IFN-γ within the supernatants were measured using a cytometric bead array. Average transduction efficiency of used lentiviral/RD114-TR CD19-CAR-NK cells was 13.1% (range 11.6–14.5%). Cytokine secretion from two donors (n = 2) are shown. (B) Cytotoxic activity of NT-NK cells, lentiviral/RD114-TR CD19-CAR-NK cells and alpharetroviral/RD114-TR CD19-CAR-NK cells against K562 and Sup-B15 cells was determined in a flow cytometry-based cytotoxicity assay after a coincubation time of 4 h at an E:T: ratio of 1:1. Average transduction efficiency of used lentiviral/RD114-TR CD19-CAR-NK cells was 13.1% (range 11.6–14.5%), of used alpharetroviral/RD114-TR CD19-CAR-NK cells was 23.4% (range 22.6–23.9%). Shown are co-incubations using NK cells of two different donors (n = 2). Shown are mean values + SD.