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Brine shrimp lethality assay (BSLA). ME was tested at 10, 2, 1, 0.5, 0.25, 0.125, 0.0625 μg/μl, while control was untreated. Cytotoxic effect of ME on shrimp nauplii was examined after 24 h as per protocol. Calculated LC50 was 0.70 ± 0.29 μg/μl. Experiments performed in quadruplicates and repeated three times. *p < 0.05; **p < 0.005; ***p < 0.001.
