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. 2020 Feb 10;10:1647. doi: 10.3389/fphar.2019.01647

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Copyright © 2020 Patti, Palmioli, Vitalini, Bertazza, Redaelli, Zorzan, Rubin, Mian, Bertolini, Iacobone, Armanini, Barollo, Airoldi, Iriti and Pezzani

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cells morphology evaluated by Wright's staining method in SW13 and H295R cells at 24 and 72 h, respectively. (A) representative pictures of SW13 and H295R cells. The arrows show apoptotic (white) or necrotic cells (black). (B, C) number of counted cells for SW13 cell line. B1: ME 0.25 μg/μl + mitotane 10μM. C1: ME 0.5 μg/μl + mitotane 10μM. D1: ME 1 μg/μl + mitotane 10μM. Treatment vs control: *p < 0.05; **p < 0.005; ***p < 0.001. At least 600 cells were counted for every experiment in 10 different fields and each experiment was repeated twice.