Figure 7.

PI3K-Akt signaling pathway mediates the effect of heterozygous p53-R280T on cell cycle and apoptosis of NPC cells. (A) Representative results (left) and statistical analyses (right) of cell cycle distribution analyzed by flow cytometry in the p53 KO CNE2 and C666-1 cells transiently transfected with p53-R280T mutation and wt p53 plasmid at a 1:1 ratio, followed by treatment with 50μM LY294002 for 12h. (B) Representative results (left) and statistical analyses (right) of cell apoptosis analyzed by flow cytometry in the p53 KO CNE2 and C666-1 cells transiently transfected with p53-R280T mutation and wt p53 plasmid at a 1:1 ratio, followed by treatment with 50 μM LY294002 for 12h. *P < 0.05; **P < 0.01; ***P < 0.001; ns, no significance. Vector, transfected with an empty vector; KO, p53 knockout; LY, LY294002.