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Primers used for real-time (RT)-PCR analysis of tumor necrosis factor (TNF)-α and interleukin (IL)-1β messenger RNA (mRNA) in HL-1 cells.
| Primer name | Sequence |
|---|---|
| TNF-α forward | TGCTCTGTGAAGGGAATGGG |
| TNF-α reverse | ACCCTGAGCCATAATCCCCT |
| IL-1β forward | ATGAAAGACGGCACACCCAC |
| IL-1β reverse | AAGGCAGAGTCTTCGGTGAG |
| GAPDH forward | CCCTTAAGAGGGATGCTGCC |
| GAPDH reverse | ACTGTGCCGTTGAATTTGCC |
