FIGURE 8.

The effects of ActD and BIX treatment on rDNA transcription initiation. (A) qRT-PCR was used to detect the incomplete 5′ETS transcripts and the mature rRNA expressions in HeLa cells after treatment with ActD for 24 h. (B) qRT-PCR was used to detect the incomplete 5′ETS transcripts and the mature rRNA expressions in HeLa cells after treatment with BIX for 48 h. Expression values were normalized to the gene GAPDH. The relative expression ratio of each sample was compared with untreated cells, the expression value of which were assigned as 1. Each experiment was repeated three times, and the average value and SD are shown. Data are expressed as *P < 0.05, **P < 0.01, and ***P < 0.001, measured by the t-test.