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. 2020 Feb 7;10:31. doi: 10.3389/fcimb.2020.00031

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Copyright © 2020 Shahid, Cavalcante, Knight, Barkema, Han, Gao and Cobo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Prototheca bovis was present at similar levels in the mammary glands of Camp^+/+ and Camp^−/− mice. (A) Mammary gland from a Camp^+/+ mouse infected with P. bovis (4 d post-infection). Abundance of Prototheca organisms within tissue sections was revealed with special stains. Prototheca are stained magenta by periodic acid-Schiff (PAS) stain (left image) and black by Grocott methenamine silver (GMS) stain (right image). Scale bar = 20 μm. (B) P. bovis was present in mammary glands at similar levels in Camp^+/+ and Camp^−/− mice, as determined by counting of sporangia per field (minimum of 10 randomly chosen microscopic fields, x40 objective magnification) and culturing 10-fold diluted homogenates on Sabouraud dextrose agar. Data are means ± SEM (n = 4/group). Ns, not significant.