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. 2020 Jan 23;10:3121. doi: 10.3389/fmicb.2019.03121

FIGURE 2.

FIGURE 2

Expression analysis of PEPCK in different strains. The strains were grown on rich media and harvested in the transition phase. (A) Western blot analysis using FE01, FE02, and FE03 to detect PEPCK-F3 under control of its endogenous promoter. (B) Western blot analysis using FE04, FE05, and FE06 to detect PEPCK-F3 under control of the synthetic promoter SP4. (C) RT-qPCR results. mRNA levels of pepck were detected in S. coelicolor M145, Δscr5239, and scr5239+ and normalized to the levels in M145. Measurements were normalized to the respective strain expressing wild type scr5239; error bars represent the standard deviation calculated from three independent experiments.