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. 2020 Jan 23;10:3121. doi: 10.3389/fmicb.2019.03121

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Copyright © 2020 Engel, Ossipova, Jakobsson, Vockenhuber and Suess.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of the interaction of scr5239 and PEPCK mRNA. (A) Predicted interaction site. The sequence of the sRNA is shown completely, that of the pepck mRNA only partially. The RBS (red) and the start codon (blue) of pepck are highlighted. The interaction site of dagA and metE is highlighted in red on the sRNA. (B) PEPCK was mutated at the predicted interaction site (PEPCmut-F3) and integrated in the genome of the M145, Δscr5239, and scr5239+. Mutated bases in the sequence are underlined. Western blot analysis using FE07, FE08, and FE09 and thus detecting PEPCmut-F3. (C) scr5239 was mutated at the predicted interaction site. Mutated bases in the sequence are underlined. The mutated and the wild type scr5239 in combination with PEPC-F3 were integrated into Δscr5239. Western blot analysis using FE10 and FE11 and thus detecting with PEPC-F3. Measurements were normalized to the respective strain expressing wild type scr5239; errors represent the standard deviation calculated from three independent experiments.