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. 2020 Feb 5;13:590. doi: 10.3389/fncel.2019.00590

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Copyright © 2020 Gao, Cheng, Wang, Jiang, Zhang, Wang, Wu, Zeng, Wang, Gao, Ma and Chai.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Blebbistatin significantly enhanced the viability of HEI-OC-1 cells after neomycin exposure. (A) Schematic diagram of blebbistatin (Ble) and neomycin addition in cell culture. (B) The survival of hair cell (HC)-like HEI-OC-1 cells cultured under the same conditions with different concentrations of blebbistatin. Scale bars = 100 μm. (C) Images of HEI-OC-1 cells stained with FDA (green) and PI (red). Scale bars = 20 μm. (D) The result of the CCK-8 assay. (E) The proportions of live and dead cells in (D). *p < 0.05, **p < 0.01, ***p < 0.001, ns, no significant.