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. 2020 Feb 5;13:590. doi: 10.3389/fncel.2019.00590

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Copyright © 2020 Gao, Cheng, Wang, Jiang, Zhang, Wang, Wu, Zeng, Wang, Gao, Ma and Chai.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Blebbistatin decreased reactive oxygen species (ROS) levels in HEI-OC-1 cells after neomycin injury. (A) The immunofluorescence intensity of Mito-SOX was increased after 2 mM neomycin treatment for 24 h compared with the undamaged controls, and Mito-SOX intensity was significantly reduced in the 2 mM neomycin + 1 μM blebbistatin group compared with the neomycin-only group. Scale bars = 20 μm. (B) Flow cytometry data showing the intensity of Mito-SOX in the control, neomycin-only, and 2 mM neomycin + 1 μM blebbistatin groups. (C) Quantification of the data in panel (B). (D) qPCR results showing the expression of the antioxidant genes Gsr, Sod1, Alox15, and Nqo1 after neomycin and blebbistatin treatment. *p < 0.05, **p < 0.01, ***p < 0.001, ns, no significant. Scale bars = 16 μm.