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. 2020 Feb 5;13:590. doi: 10.3389/fncel.2019.00590

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Copyright © 2020 Gao, Cheng, Wang, Jiang, Zhang, Wang, Wu, Zeng, Wang, Gao, Ma and Chai.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Blebbistatin decreased ROS levels in cochlear HCs after neomycin injury. (A) The immunofluorescence intensity of Mito-SOX was increased after 0.5 mM neomycin treatment for 12 h compared with the undamaged controls, and Mito-SOX intensity was significantly reduced in the 0.5 mM neomycin + 1 μM blebbistatin group compared with the neomycin-only group. (B) Quantification of the numbers of Mito-SOX/myosin7a double-positive cells in A. **p < 0.01, ***p < 0.001. Scale bars = 16 μm.