Figure 1.

(A) Mid-modiolar cross-section through a hematoxylin and eosin (H&E)-stained guinea pig cochlea, depicting its four spiraling turns. The microcannula is positioned through a cochleostomy adjacent to the round window to enable slow cochlear perfusion through scala tympani (ST). (B) Experimental timelines for control and TNF-α experiments vs. prevention experiments. The timelines are identical aside from the subcutaneous (SC) injection of a TNF-α-blocker (etanercept) prior to perfusion of TNF-α in prevention experiments. Green arrows indicate time points of hearing tests (CAPs and DPOAEs), black arrows indicate time point of post mortem immunohistochemistry (IHC).