Figure 5.

Anti-tumor efficacy of α-Al₂O₃-UPs vaccine in 4T1 tumor-bearing mice. (A) The immunization schedule for α-Al₂O₃-UPs vaccination in an established 4T1 tumor model. (B) The level of IFN-γ in the serum of tumor-bearing mice after treatment. (C, D) The tumor volume and the percent survival time of tumor burden mice with different treatments (n=6 per group). (E) The immunization protocol for combination therapy for the 4T1 tumor-bearing model. (F) The level of IFN-γ in the serum of tumor-bearing mice after treatment. (G, H) The tumor volume and the percent survival time of tumor-bearing mice with combination therapy (n=6 per group). Data (mean ± SD) are representative of three independent experiments results. **, P < 0.01; ****, P < 0.0001; ns, not significant, by One-way ANOVA with the Tukey-Kramer multiple test, two-tailed unpaired t-test or Mann–Whitney U-test. Kaplan-Meier survival curves were assessed by the Log-rank Mantel-Cox test.