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. 2020 Feb 13;13:1303–1310. doi: 10.2147/OTT.S233495

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© 2020 Zhang et al.

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Knockdown of KLF16 inhibits prostate cancer (PCa) cell growth in vitro and in vivo. PC3 and 22RV1 cells were infected by a lentivirus with a short hairpin RNA (shRNA) targeting KLF16 or a control sequence. (A) The efficiency of the shRNA in PCa cells was confirmed by Western blotting. The semi-quantitation of Western blotting was shown under the pictures. (B) PC3 and 22RV1 cells expressing shRNA targeting KLF16 or a control sequence were cultured in 96-well plates for 6 days. The MTT assay was conducted to determine viability of the indicated cells (t-test; *p < 0.05; ***p < 0.001). (C) PC3 and 22RV1 cells expressing their respective shRNAs were examined using a colony formation assay. (D) The numbers of colonies for each group were counted and the results are shown (t-test; ***p < 0.001). (E) The mice with xenograft were shown after implanting PC3 cells 6 weeks later. (F) The weight of the xenograft tumors was calculated. Error bars represent mean ± SD (Mann–Whitney, ** p < 0.01, n = 6).