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. 2020 Feb 13;13:1321–1330. doi: 10.2147/OTT.S239407

Figure 2.

Figure 2

DUSP9 inhibits ccRCC cell proliferation and migration in vitro. (A, B) (A) Fluorescence labeling and (B) Western blot analysis confirmed the successful overexpression of DUSP9 in 769-P and 786-O cells. (C) CCK-8 viability assay demonstrated the overexpression of DUSP9 to significantly reduce the proliferation of 786-O and 769-P cells (Repeated-measures analysis of variance, P < 0.001). (D) Transwell assay and (E) wound-healing assay confirmed the overexpression of DUSP9 to inhibit migration in 769-P and 786-O cells; *p < 0.05, **p < 0.01, ***p < 0.001. All data were representatives of three independent experiments.

Abbreviations: DUSP9, dual specificity phosphatase 9; NC, negative control; HA, HA protein tag; CCK-8, Cell Counting Kit-8.