Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifier | Additional information |
|---|---|---|---|---|
| Biological sample (Malassezia globosa) | CBS7966 | CBS | Wild-type strain | |
| Biological sample (Malassezia slooffiae) | CBS7956 | CBS | Wild-type strain | |
| Biological sample (Malassezia furfur) | CBS14141 | CBS | Wild-type strain | |
| Biological sample (Malassezia sympodialis) | ATCC42132 | ATCC | Wild-type strain | |
| Biological sample (Saccharomyces cerevisiae) | BY4741 | Wild-type strain (MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0) |
||
| Genetic reagent (Malassezia sympodialis) | MSY001 | This study | M. sympodialis (ATCC42132) cells expressing GFP-Mtw1 | |
| Genetic reagent (Malassezia furfur) | MF001 | This study | M. furfur (CBS14141) cells expressing CENP-A-3xFLAG | |
| RecombinantDNA reagent | Plasmid pGI3 | (Ianiri et al., 2017b) | Vector backbone for Agrobacterium-mediated transconjugation | |
| RecombinantDNA reagent | Plasmid pAIM1 | (Ianiri et al., 2016) | Plasmid with NAT marker, used as template for PCR | |
| RecombinantDNA reagent | Plasmid pVY7 | (Kozubowski et al., 2013) | Plasmid with GFP, used as template for PCR | |
| RecombinantDNA reagent | Plasmid pMHR04 | This study | Construct used to tag MsyMtw1 with GFP tag at the amino-terminus | |
| RecombinantDNA reagent | Plasmid pMF1 | This study | Construct used to tag MfCENP-A with 3xFLAG tag at the carboxyl terminus | |
| Antibody | Anti-GFP (mouse monoclonal) | Roche | 11814460001 | IF (1:100) WB (1:3000) |
| Antibody | Anti-PSTAIRE (mouse monoclonal) | Abcam | Cat. no. 10345 | WB (1:5000) |
| Antibody | Anti-mouse IgG HRP (goat polyclonal) | Abcam | Cat. no. ab97023 | WB (1:10,000) |
| Antibody | Anti-H3 (rabbit polyclonal) | Abcam | Cat. no. ab1791 | ChIP (5 μL per 500 μL IP fraction) |
| Antibody | Anti-H4 (rabbit polyclonal) | Abcam | Cat. no. ab10158 | ChIP (5 μL per 500 μL IP fraction) |
| Antibody | Anti-FLAG (M2) (mouse monoclonal) | Sigma | Cat. no. F1804 | WB (1: 2500) |
| Other | GFP-trap beads | ChromoTek | Cat. no. gta-20 | ChIP (20 μL per 500 μL fraction) |
| Other | Blocked agarose beads | ChromoTek | Cat. no. bab-20 | ChIP (20 μL per 500 μL fraction) |
| Other | Protein-A sepharose beads | Sigma | Cat. no. P3391 | ChIP (20 μL per 500 μL fraction) |
| Other | M2 anti-FLAG affinity gel | Sigma | Cat. no. A2220 | ChIP (20 μL per 500 μL fraction) |
| Other | Lysing enzymes from Trichoderma harzianum |
Sigma | Cat. no. L1412 | Enzyme to prepare spheroplasts |
| Other | Zymolyase 20T | MP biomedicals | Cat. no. 320921 | Enzyme to prepare spheroplasts |
| Other | Chitosanase | Sigma | Cat. no. C0794 | Enzyme to prepare spheroplasts |
| Other | SensiFAST SYBR No ROX Kit | Bioline | Cat. no. BIO-98020 | qPCR assay reagent |
| Chemical compound, drug | 2-mercaptoethanol | HiMedia | Cat. no. MB041 | |
| Software, algorithm | Geneious 9.0 | Biomatters Ltd. | ||
| Software, algorithm | SyMap | (Soderlund et al., 2011) | ||
| Software, algorithm | PhylloGibbs-MP | (Siddharthan, 2008) | ||
| Software, algorithm | Circos | (Krzywinski et al., 2009) | ||
| Software, algorithm | Satsuma | (Grabherr et al., 2010) | ||
| Software, algorithm | Easyfig | (Sullivan et al., 2011) | ||
| Software, algorithm | GET_HOMOLOGUES | (Contreras-Moreira and Vinuesa, 2013) | ||
| Software, algorithm | IQ-TREE v1.6.5 | (Nguyen et al., 2015) | ||
| Software, algorithm | iTOL v4.3.3 | (Letunic and Bork, 2019) | ||
| Software, algorithm | SMRTPortal v2.3 | PacBio, Menlo Park, CA, USA | ||
| Software, algorithm | Fiji | National Institutes of Health | ||
| Software, algorithm | Illustrator | Adobe Systems | ||
| Software, algorithm | Excel | Microsoft | ||
| Software, algorithm | Word | Microsoft | ||
| Sequence-based reagent | List of primers used in this study | Sigma | In Supplementary file 1 |