Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (M. musculus) |
Tgfbr2f/f | (Chytil et al., 2002) | NA | NA |
| Genetic reagent (M. musculus) |
ScxCre | (Blitz et al., 2013) | NA | NA |
| Genetic reagent (M. musculus) |
RosaCreERT | (Hameyer et al., 2007) | NA | NA |
| Genetic reagent (M. musculus) |
ScxGFP | (Pryce et al., 2007) | NA | NA |
| Genetic reagent (M. musculus) |
Ai14 Rosa26-tdTomato (RosaT) | (Madisen et al., 2010) | NA | NA |
| Recombinant DNA reagent | pAAV1-FLEX-Tgfbr2-V5 | GenScript | This paper | NA |
| Recombinant DNA reagent | pAAV1-Tgfbr2-FLAG | GenScript | This paper | NA |
| Antibody | Rat anti-CD34 (Clone RAM34) | BD Biosciences | Cat# 553731
RRID:AB_395015 |
IF(1:200), Antigen retrieval |
| Antibody | Rat anti-CD44 (Clone IM7) | BD Biosciences | Cat# 550538
RRID:AB_393732 |
IF(1:40), Pre-treated with cold acetone for 10 min at −20°C |
| Antibody | Rabbit anti-FLAG (DYKDDDDK) | Thermo Fisher Scientific | Cat# 740001
RRID:AB_2610628 |
IF(1:200), Antigen retrieval |
| Antibody | Rat anti-FLAG (DYKDDDDK) | Novus Biologicals | Cat# NBP1-06712SS
RRID:AB_1625982 |
IF(1:100), Antigen retrieval |
| Antibody | Goat anti-Sca-1/Ly6 | R and D Systems | Cat# AF1226
RRID:AB_354679 |
IF(1:80) |
| Antibody | Rat anti-Sca-1/Ly6 | R and D Systems | Cat# MAB1226
RRID:AB_2243980 |
IF(1:50) |
| Antibody | Goat anti-tenomodulin (Clone C-20) |
Santa Cruz Biotechnology | Cat# sc-49324
RRID:AB_2205971 |
IF(1:50), Antigen retrieval |
| Antibody | Rabbit anti-TGFβ type II receptor | Bioworld Inc | Cat# BS1360
RRID:AB_1663474 |
IF(1:250) |
| Antibody | Rabbit anti-V5 | Abcam | Cat# ab206566
RRID:AB_2819156 |
IF(1:500), Antigen retrieval |
| Antibody | Rat anti-V5 | Abcam | Cat# ab206570
RRID:AB_2819157 |
IF(1:500), Antigen retrieval |
| Antibody | Cy5 donkey anti-goat secondary | Jackson ImmunoResearch | Cat# 705-175-147
RRID:AB_2340415 |
IF(1:500) |
| Antibody | AlexaFluor647 donkey anti-rabbit secondary | Jackson ImmunoResearch | Cat# 711-607-003
RRID:AB_2340626 |
IF(1:400) |
| Antibody | Cy3 donkey anti-rabbit secondary | Jackson ImmunoResearch | Cat# 711-166-152
RRID:AB_2313568 |
IF(1:800) |
| Antibody | AlexaFluor647 donkey anti-rat secondary | Jackson ImmunoResearch | Cat# 712-606-153
RRID:AB_2340696 |
IF(1:800) |
| Antibody | Cy3 donkey anti-rat secondary | Jackson ImmunoResearch | Cat# 712-166-150
RRID:AB_2340668 |
IG(1:800) |
| Commercial assay or kit | In situ cell death detection kit | Roche | Cat# 12156792910 | Follow the manufacturer’s instruction |
| Commercial assay or kit | Click-iT EdU kit | Life Technologies | Cat# C10340 | Follow the manufacturer’s instruction |
| Other | DAPI stain | Thermo Fisher Scientific |
D1306
RRID:AB_2629482 |
1 μg/ml |
Note:.
* Antigen retrieval: Incubated with warm citrate buffer (10 mM sodium citrate with 0.05% Tween 20, pH 6) at 550W, 50°C for 5 min using a PELCO BioWave.