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. Author manuscript; available in PMC: 2020 Feb 17.
Published in final edited form as: Nat Med. 2019 Mar 4;25(4):612–619. doi: 10.1038/s41591-019-0356-z

Fig. 2. Pyroglutamate formation occurs early in the CD47 protein life-cycle and is fully dependent on QPCTL.

Fig. 2

a, SDS-PAGE analysis of αhCD47-B6H12 (B) or αhCD47-CC2C6 (C) immunoprecipitates from CD47-HA-overexpressing WT or QPCTL KO (cl4.1) A375 melanoma cells after a 0, 1, 2 or 4 hours (h) chase period following a 30’ 35S methionine/cysteine labelling.

b, SDS-PAGE analysis of αhCD47-B6H12 (B) or αhCD47-CC2C6 (C) immunoprecipitates from CD47-HA-overexpressing WT or QPCTL KO (cl4.1) A375 melanoma after a 0 or 30’ chase following a 10’ 35S methionine/cysteine labelling.

Data are representative of two (a, b) independent experiments with similar results. Blot images are cropped to show the relevant bands, and molecular mass markers are indicated (in kD). See Source Data for the uncropped western blots.

OE, over expression; B, αhCD47-B6H12; C, αhCD47-CC2C6.