Extended Data Figure 9. Force application on other members of the PlxnD1 mechano-complex does not elicit a mechanotransduction response.
Mouse ECs were incubated with (a) anti-VEGFR2 or (b) anti-NRP1 antibody-coated beads and subjected to force (10pN) for the indicated time periods. Phosphorylation of Akt and ERK1/2 was determined by western blotting and quantified using Image Studio Lite Ver 5.2, n=3 biological repeats. The data represent mean±SEM.