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. Author manuscript; available in PMC: 2020 Aug 5.
Published in final edited form as: Nature. 2020 Feb 5;578(7794):290–295. doi: 10.1038/s41586-020-1979-4

Extended Data Figure 1. Knockout/knockdown of PlxnD1 and other genes in ECs.

Extended Data Figure 1

(a-f) ECs were either isolated from PlxnD1fl/fl and PlxnD1iECKO mice, or treated with siRNAs to knockdown PlxnD1, NRP1, Piezo1 and Gαq/11. Knockdowns/knockouts were confirmed by western blotting, using GAPDH as a loading control (g) PlxnD1 was knocked down in mouse ECs using a pool of siRNAs, followed by infection with an adenovirus expressing either β-galactosidase (LacZ), WT or mutant PlxnD1. Protein levels were normalised against the housekeeping gene GAPDH. KD=Mean knockdown efficiency based on n=3; KO= Mean knockout efficiency based on n=3.