Figure 3.

The Induction of lncRNA ENSMUST00000160470 (Mbd2-AL1) by I/R Is Inhibited by Mbd2 siRNA in RGCs

RGCs were transfected with 50 nM Mbd2 siRNA or scramble for 24 h and then left treated or untreated with ischemia for 2 h and reperfusion for another 2 h. (A) Representative heatmap of lncRNA microarray analysis. (B) The amount of each lncRNA from the scramble/I/R group divided by the amount of the scramble group to calculate the up-fold change and from the Mbd2 siRNA/I/R group divided by the scramble/I/R group getting the down-fold change. (C) Quantitative real-time PCR analysis of ENSMUST00000160470 and ENSMUST00000123262. The value of each lncRNA was normalized by glyceraldehyde 3-phosphate dehydrogenase (GAPDH), an internal control. Data were expressed as mean ± SEM of five independent experiments. #p < 0.05 versus the scramble group; *p < 0.05 versus the scramble/I/R group. (D) The levels of lncRNA ENSMUST00000160470 (Mbd2-AL1) induced by I/R treatment at indicated time points (0 h, 2 h, and 4 h). Data were expressed as mean ± SEM of five independent experiments. #p < 0.05 versus the 0/0 group.