Figure 6.

miR-188-3p Is a Direct Target of lncRNA Mbd2-AL1

(A) The sequence-alignment analysis revealed that Mbd2-AL1 RNA contains a complementary site to miR-188-3p. (B) Luciferase activity, assessed 48 h after cotransfection of lncRNA Mbd2-AL1 WT or Mbd2-AL1 MUT plasmid with miR-188-3p mimic or scramble. (C) Knockdown of lncRNA Mbd2-AL1 increases the expression level of miR-188-3p. RGCs were transfected with 50 nM Mbd2-AL1 siRNA and then left treated or untreated with ischemic stress for 2 h and reperfusion for 2 h. (D) Overexpression of Mbd2-AL1 induces the reduction of miR-188-3p expression level. RGCs were transfected with 50 nM Mbd2-AL1 plasmid and then left treated or untreated, as described in (C). miR-188-3p expression levels were analyzed by quantitative real-time PCR in (C) and (D). (E) The intracellular colocalization of Mbd2-AL1 and miR-188-3p in RGCs, with or without I/R treatment. Data were expressed as mean ± SEM of five independent experiments. #p < 0.05 versus the Mbd2-AL1/scramble or scramble group, *p < 0.05 versus Mbd2-AL1 WT/miR-188-3p mimic or the scramble/I/R group.