Figure 7.

The I/R-Induced RGC Apoptosis Is Suppressed by the Overexpression of miR-188-3p

RGCs were transfected with 50 nM miR-188-3p mimic or scramble, 24 h before I/R of 2/2 h. (A and B) Overexpression of miR-188-3p prevents RGC apoptosis that was induced by I/R. Apoptosis was analyzed by flow cytometry. (C) The expression level of miR-188-3p during I/R treatment or miR-188-3p mimic transfection. Quantitative real-time PCR is used to detect the miR-188-3p level. The value of the microRNA was normalized by the level of U6, an internal control. (D–F) The expression of cleaved caspase3 and caspase3 were analyzed by western blotting. The data were expressed as mean ± SEM of five independent experiments. #p < 0.05 versus the scramble group; *p < 0.05 versus scramble/I/R group.