Figure 9.

Mbd2-AL1 Mediates I/R-Induced RGC Apoptosis by Targeting miR-188-3p

RGCs were transfected with 50 nM Mbd2-AL1 siRNA or cotransfected with Mbd2-AL1 siRNA and miR-188-3p inhibitor, 24 h before I/R of 2/2. (A and B) The deletion of Mbd2-AL1 attenuates the apoptosis induced by I/R injury, and this effect was reversed by miR-188-3p knockdown. Apoptosis is analyzed by flow cytometry. (C and D) The levels of lncRNA Mbd2-AL1 and miR-188-3p were analyzed by quantitative real-time PCR. (E–H) The expression levels of cleaved caspase3, caspase3, and Traf3 were analyzed by western blotting. The data were expressed as mean ± SEM of five independent experiments. #p < 0.05 versus the scramble group; ^p < 0.05 versus the scramble/I/R; *p < 0.05 versus Mbd2-AL1 siRNA /I/R group.