Figure 3.

Ionizing radiation induces cell shape changes. (A) Representative images of F-actin staining indicate cobblestone-like shape in control (left) and elongated shape in irradiated cells (right) (scale bars = 20 μm). (B) Segmented images of F-actin staining (as shown in A) were used to calculate aspect ratio (AR = length of long axis/length of short axis). Mean aspect ratio, mean (AR), was calculated in four fields of view. Error bars represent the standard error of the mean from five independent experiments (n = 5).