Table 1.
Markers for murine pulmonary macrophage and dendritic cell subsets.
| Marker | Alveolar Mac | Interstitial Mac | Ly6c+ Mo-like Mac | Ly6c- Mo-like Mac | Inflam mono | CD103 DC | CD11b DC |
|---|---|---|---|---|---|---|---|
| CD45 | + | + | + | + | + | + | + |
| CD11b | − | + | + | + | hi | − | + |
| CD11c | + | lo | +/− | +/− | − | + | + |
| CD68 | hi | lo | − | − | − | hi | hi |
| MHC II | +/− | + | − | − | − | + | + |
| Siglec F | hi | − | − | − | − | − | − |
| F4/80 | + | + | + | + | + | − | − |
| CD14 | − | lo | − | − | |||
| CD24 | − | − | − | − | − | + | + |
| CD103 | − | − | − | − | − | + | − |
| Ly6c | − | − | + | − | hi | − | − |
| Ly6G | − | − | − | − | − | ||
| CD115 | − | − | − | − | + | − | − |
| CCR2 | − | − | + | + | hi | − | −/+ |
Murine pulmonary macrophages/monocytes are divided into 5 subsets and pulmonary conventional DCs are divided into 2 subsets based on cell surface expression of multiple markers (Guilliams et al., 2013; Misharin et al., 2013; Zaynagetdinov et al., 2013; Yang et al., 2014; Kopf et al., 2015; Hey et al., 2016; Menezes et al., 2016; Gibbings et al., 2017).