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. 2020 Feb 11;7:396. doi: 10.3389/fcell.2019.00396

FIGURE 6.

FIGURE 6

Sevoflurane anesthesia reduces binding of CypD and ANT. (A) CypD mainly locates in isolated mitochondria, but not cytosol, and its level can be increased in the isolated mitochondria of WT NPCs following the sevoflurane anesthesia. (B) RT-PCR shows that sevoflurane anesthesia does not significantly increase the mRNA levels of CypD as compared to the control condition in WT NPCs (P = 0.802, Student’s t test, N = 3). (C) Co-immunoprecipitation studies show that sevoflurane anesthesia reduces the binding of CypD with ANT as compared to the control condition in H4 human neuroglioma cells. (D) Immunocytochemistry images show that there are both CypD bound with ANT (yellow arrow) and CypD separated with ANT (white arrow) in the H4 human neuroglioma cells following sevoflurane anesthesia. Specifically, the green dots demonstrated CypD (D, top left panel), and the red dots showed ANT (D, top right panel). The white arrows in the bottom two panels of D indicated the CypD that did not bind to ANT, and the yellow arrows in the bottom panels of D revealed the CypD that bonded to ANT.