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. 2020 Feb 11;8:50. doi: 10.3389/fbioe.2020.00050

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Copyright © 2020 Bolat, Islek, Demir, Yilmaz, Sahin and Ucisik.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Apoptotic cell death of HCT116 cells upon treatment with 25 μM CurcuEmulsome, 7 μM PiperineEmulsome and their combinations for 48 and 72 h. (A) Representative histogram data of Annexin V-FITC/PI flow cytometer analysis, and (B) apoptotic cell percentage were presented. (C) Fold increase of relative caspase3/mRNA expression using real time PCR in HCT116 cells treated with 25 μM CurcuEmulsome, 7 μM PiperineEmulsome and their combinations. The data represents three independent experiments and P ≤ 0.05 (*) shows significant difference from respective control.