Table 5.
Primers used in this work.
| Gene | Primer (5′-3′) | size (bp) | ref. |
|---|---|---|---|
| 16s rRNA |
F-AGT TTG ATC MTG GCT CAG R-GGA CTA CHA GGG TAT CTA AT |
797 | 49 |
| blaTEM |
F-ATG AGT ATT CAA CAT TTC CG R-CCA ATG CTT AAT CAG TGA GG |
858 | 50 |
| blaSHV |
F-ATG CGT TAT ATT CGC CTG TG R-AGC GTT GCC AGT GCT CGA TC |
862 | 50 |
| Universal blaCTX-M |
F-5′-SCS ATG TGC AGY ACC AGT AA R-5′-CCG CRA TAT GRT TGG TGG TG |
554 | 51,52 |
| blaCTX-M-1 |
F-5′-AAA AAT CAC TGC GCC AGT TC R-5′-AGC TTA TTC ATC GCC ACG TT |
415 | 51,52 |
| blaCTX-M-2 |
F-5′-CGA CGC TAC CCC TGC TAT T R-5′-CCA GCG TCA GAT TTT TCA GG |
552 | 51,52 |
| blaCTX-M-8 |
F- 5′-TCG CGT TAA GCG GAT GATGC R-5′-AAC CCA CGA TGT GGG TAG C |
666 | 51,52 |
| blaCTX-M-9 |
F-5′-CAA AGA GAG TGC AAC GGA R-TG 5′-ATT GGA GGT TCA TCA CC |
205 | 51,52 |
| blaCTX-M-25 |
F-5′-GCA CGA TGA CAT TCG GG R-5′-AAC CCA CGA TGT GGG TAG C |
327 | 51,52 |
| blaCTX-M-15 |
F-5′-ATAAAACCGGCAGCGGTG R-5′-GAATTTGACGATCGGGG |
500 | 51,52 |
| chuA |
F-5′- GACGAACCAACGGTCAGGAT R-5′- TGCCGCCAGTACCAAAGACA |
279 | 12 |
| yjaA |
F-5′- TGAAGTGTCAGGAGACGCTG R-5′- ATGGAGAATGCGTTCCTCAAC |
211 | 12 |
| TspE4C2 |
F-5′- GAGTAATGTCGGGGCATTCA R-5′- CGCGCCAACAAAGTATTACG |
152 | 12 |
| arpA |
5′-AACGCTATTCGCCAGCTTGC-3′ 5′-TCTCCCCATACCGTACGCTA-3′ |
400 | 12 |