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. 2020 Feb 17;10:2762. doi: 10.1038/s41598-020-59628-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Echocardiography assessment. (a) Left ventricular internal diameter during diastole (LVIDd) and (b) during systole (LVIDs). (c) LV mass. (d) Representative 40x sections of Masson’s trichrome stained cardiac tissue from male rats. (e) Male collagen area (blue staining) as a % of total tissue area. (f) Intraventricular septum during diastole (IVSd) and (g) during systole (IVSs). (h) Left ventricular posterior wall thickness during diastole (LVPWd) and (i) during systole (LVPWs). (j) Ejection fraction (EF). Statistical analysis was performed using the repeated measures mixed procedure in SAS and data are presented as Least Squares Mean (LSM) ± SEM (*P < 0.05).