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. 2020 Feb 17;11:926. doi: 10.1038/s41467-020-14665-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a A schematic depiction of Homo sapiens tRNA-Arg^TCG, showing identified sequences that map uniquely to this isoacceptor (yellow for unmodified and orange for modified residues). Total coverage is 52%. Two different modification variants were observed at U34 (wobble position) and C40. b Aggregated coverage of the consensus tRNA sequence by oligonucleotides identified in the human tRNA dataset. Some oligonucleotide positions in long tRNAs (tRNA-Leu, tRNA-SeC, tRNA-Ser) were adjusted to fit the consensus sequence. Complementary regions in the acceptor stem (orange), D-stem (green), anticodon stem (blue) and T-stem (red) are highlighted. D D-loop, Ac anticodon loop, V variable region, T T-loop.