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. 2020 Feb 17;10:2734. doi: 10.1038/s41598-020-59516-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Summaries of fold-change and abundance estimate differences among RNA-seq pipelines. (a) Large inter-pipeline differences in fold change estimates for the same sample pairs among discordant genes. Shown are the maximum and minimum fold-difference estimates among pipelines for each discordant gene across all sample pairs. Heatmaps showing the number of discordant samples per gene in TCGA (b) and GTEx (c) data. Each row represents one discordant gene, each column represents a comparison between two pipelines (as labeled).