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Cytotoxicity of silibinin or valsartan in vitro. (A) Chemical structure of silibinin. (B) HK-2 cells were treated with 0, 15, 30, 60 and 90 μM silibinin for 72 hrs, and cell viability was determined by CCK-8 assay. (C) HK-2 cells were treated with 0, 10, 20, 30 and 50 μM valsartan for 72 hrs, and cell viability was determined by CCK-8 assay. (D) HK-2 cells were treated with silibinin plus valsartan for 72 hrs, and cell viability was detected by CCK-8 assay. **P<0.01 vs 0 μM group.
