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. 2020 Feb 17;10:2708. doi: 10.1038/s41598-020-59622-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Western blot analysis of PSA from seminal plasma and cancer cells. (a) Seminal PSA with and without PNGF treatment. (b) PSA from LNCaP in Con A (−) and (+) fraction with and without PNGF treatment. (c) Immunoprecipitated PSA in Con A (−) and (+ fraction from CTOS. (d) Immunoprecipitated PSA in Con A (−) fraction from CTOS with and without PNGF treatment. The position of each high molecular and low molecular PSA was indicated by closed and open triangle, respectively. Full-length blots are presented in Supplementary Fig. S4.