Figure 3.

NOD1 stimulation provokesin vivohepatic metastasis of colon cancer cells. (A) Representative murine liver samples with metastasis for each condition are shown. Compared to DMSO control (left), C12-iE-DAP (2,000 ng/mL) (middle) stimulation of MC38 colon cancer augments their metastasis. This augmentation is abrogated with ML130 (10 μmol/L) co-incubation (right). (B) Results from 2 independent experiments (n = 7 mice/condition) are quantified by counting the number of surface liver nodules post-necropsy. Only 8/14 mice in the DMSO group, 6/14 mice in the C12 group, and 9/14 mice in the ML130 + C12 group survived until 21 days post intrasplenic cancer cell injection for inclusion in the figure. Mice that died prior to the endo-point did not have any liver metastasis on necrosectomy. M and SEM are shown. All comparisons are made with respect to the DMSO control. Only significant comparisons are labelled. “***” denotes P < 0.0001