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. 2020 Jan 9;102(4):501–515. doi: 10.1007/s11103-019-00961-4

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© The Author(s) 2020

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Fig. 4 — Map-based cloning of ES5 gene. a Preliminary mapping of ES5 between the SSR markers RM 3486 and RM 3664 on the long arm of chromosome 5. bES5 gene was fine mapped to an interval of 37.57 kb region by 1437 mutant individuals. c Six putative ORFs were annotated in the 37.57 kb region, and gDNA sequencing revealed that ORF2 had one base substitution from G-to-A and creation of a premature stop codon (d, e)