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. 2020 Feb 11;11:20. doi: 10.3389/fimmu.2020.00020

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Copyright © 2020 Bonezi, Cataneo, Branquinho, Silva, Gonzalez-Dias, Pereira, Ferreira, Nakaya, Campa, Wowk and Silveira.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Dengue virus promotes the B cell differentiation into antibody-secreting cells (ASCs) in vitro disregarding pre-existing DENV immunity. (A) Representative FACS data displaying the percentage of cells with the ASC phenotype at day 7 of culture. (B,C) Percentage and absolute number of ASCs quantified through FACS for different PBMC cultures derived from 19 healthy donors. (D) Representative data obtained from 3.3 × 10⁴ PBMCs harvested at day 7 of culture and added per well of ELISPOT plates to enumerate IgG-secreting cells in each culture condition. (E) Magnitude of IgG-secreting cells detected per 10⁶ PBMCs through ELISPOT. (F) Heatmap based on Z scores derived from ELISPOT data for each culture condition. Green boxes represent the PBMC donors that presented pre-existing immunity to DENV.