Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Feb 11;11:20. doi: 10.3389/fimmu.2020.00020

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2020 Bonezi, Cataneo, Branquinho, Silva, Gonzalez-Dias, Pereira, Ferreira, Nakaya, Campa, Wowk and Silveira.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

DENV-mediated ASC differentiation is dependent on the physical contact between CD19+ B cells and the remaining PBMCs. (A) CD19+ B cells were isolated from PBMCs (n = 5) and cultured without physical contact with the flow through (CD19– cells) (in a 1:10 ratio, respectively) in transwell plates. CD19+ B cells were harvested at day 7 of culture and had the ASC phenotype (B–D) and function (E,F) assessed. (F) A total of 2.2 × 10⁴ CD19+ cells were added per well, except for the Mitogen culture (2.2 × 10³ CD19+ cells).